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GDSNext RNA First Strand Synthesis Module K020-A/K020-B

Products & Applications > NGS Library Prep Module > GDSNext RNA First Strand Synthesis Module K020-A/K020-B

GDSNext RNA First Strand Synthesis Module K020-A/K020-B

Price: $194.00~600.00
K020-A(24 rxns) K020-B(96 rxns)

Product Name

GDSNext RNA First Strand Synthesis Module

Cat. No./Spec.

K020-A/24 rxns; K020-B/96 rxns

Product Description

The GDSNext RNA First Strand Transcription Module is an optimized RNA first-strand transcription module that can transcribe a wide range of input RNA into cDNA using random primers.

This module can be used in conjunction with the GDSNext Directional RNA Second Strand Synthesis Module (# K021) for specific RNA second-strand synthesis or the GDSNext Non-Directional RNA Second Strand Synthesis Module (# K022) for non-specific RNA second-strand synthesis. These workflows are compatible with poly(A) mRNA isolation or ribosomal RNA depletion and enable high-yield preparation of high-quality libraries from 10 ng to 1 µg of total RNA.

Components

Component

K020-A (24 rxns)

K020-B (96 rxns)

GDSNext First Strand Synthesis Enzyme Mix

192 µL

768 µL

GDSNext First Strand Synthesis Reaction Buffer

48 µL

192 µL

Random Primers

48 µL

192 µL

GDSNext Strand Specificity Reagent

192 µL

768 µL

Storage Condition & Shelf Life

All reagents should be stored at -20°C. The product is valid for 18 months.

Application

For the synthesis of the first-strand cDNA of RNA in NGS library preparation.



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