Product Introduction
GDS Fast Fragmentation & End Prep Module is designed for library construction of high-throughput sequencing platform. The module combines fragmentation of intact DNA, end repair and dA tailing in 8 minutes. Not only the reaction time is minimized, but also the cutting efficiency of trace template is increased. 100 pg ~ 500 ng DNA template can be efficiently fragmented with 5’ phosphorylated 3' dA-tailed ends. This product contains the enzymes and buffers required for the reaction, easy to use, and compatible with automatic library preparation equipment.
Application
Fragmentation, end repair and dA-tailing in dsDNA library construction.
Features
Rapid and simultaneous completion of DNA fragmentation, end repair, and dA tailing
Components
Component | K032-A (24 rxns) | K032-B (96 rxns) |
GDS FEP Enzyme Mix | 240 μl | 2×480 μl |
GDS FEP Buffer | 120 μl | 480 μl |
Storage Condition
All reagents should be stored at -20°C.