LD DNA MARKER of GDSBIO
Product Name
LD 1kb Ladder Plus
Cat. No./Spec.
LM1191/60 preps, LM1192/60 preps×3
Description
LD 1kb Ladder Plus is an ideal DNA Ladder for determining the size of double-stranded DNA (dsDNA) from 100 to 10,000 base pairs. The ladder consists of 15 linear double-stranded fragments. The 500bp and 3,000bp fragments are present at increased intensity to allow easy identification. All fragments are precisely quantified and mixed during the production. For 5 µl loading, all fragments except 500bp and 3,000bp are 4ng. The 500bp and 3,000bp fragments are 10ng. This ladder is pre-mixed with loading dye and is ready to use.
This product is an LD series DNA Marker, suitable for use with non-toxic nucleic acid dyes during the pre-staining of agarose gels.
Application
• agarose gel electrophoresis, AGE
• Non-denaturing polyacrylamide gel electrophoresis (PAGE)
• pulsed field gel electrophoresis, PFGE
Features
• Clear and bright bands for accurate molecular weight determination
• Stable quality allows for use at room temperature
• Multiple ranges available with 23 classic series and 18 LD series options to suit different gel dyes
• Two Marker series are suitable for different types of gel dyes, and the strips are not easily deformed
Components
Component | LM1191 (60 preps) | LM1192 (60 preps×3) |
LD 1kb Ladder Plus | 300 µL | 300 µL×3 |
Recommended Amount
5 µl/lane
Recommended Electrophoresis Condition
5 µl/lane, 8 cm 1% Agarose Gel, 1×TAE, 7 V/cm, 45min.
Bands (bp)
100、200、300、400、500、700、1,000、1,500、2,000、3,000、4,000、5,000、6,000、8,000、10,000.
Concentration
Total concentration, 14.4 ng/µl
Typical bands, 10 ng/5µl
Other bands, 4 ng/5µl
Storage Condition
Store unopened at room temperature or 4 ̊C for 2 years.
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