CLASSIC DNA MARKER of GDSBIO
Product Name
λ DNA/HindⅢ
Cat. No./Spec.
M1201/50 μg, M1202/50 μg×5
Description
The λ DNA/HindⅢ is a DNA marker, consisting of 8 fragments generated by the digestion of lambda DNA (λ DNA) with the HindⅢ enzyme. It is suitable for use as molecular weight standards for agarose gel electrophoresis. λ DNA/HindⅢ is pre-mixed with loading buffer and is ready to use.
Application
• agarose gel electrophoresis, AGE
• Non-denaturing polyacrylamide gel electrophoresis (PAGE)
• pulsed field gel electrophoresis, PFGE
Features
• Clear and bright bands for accurate molecular weight determination
• Stable quality allows for use at room temperature
• Multiple ranges available with 23 classic series and 18 LD series options to suit different gel dyes
• Two Marker series are suitable for different types of gel dyes, and the strips are not easily deformed
Recommended Amount
2-5 µl/lane
Recommended Electrophoresis Condition
5 µl/lane, 8 cm 0.7% Agarose Gel, 1×TAE, 7 V/cm, 45min.
Bands (bp)
125、564、2,027、2,322、4,361、6,557、9,416、23,130.
Storage Condition
Stable for 3 months at RT, for long-time storage, please store at -20 ̊C.
Attention!
The cohesive ends of fragments 1 and 4 may cause formation of extra band 27,491 bp.
The fragments may be separated by heating to 65 ̊C for 3 minutes before loading the sample on the gel.
Components
Component | M1201 (50 µg) | M1202 (50 µg×5) |
λDNA /Hind Ⅲ | 1250 µL | 1250 µL×5 |