500bp Ladder DNA Marker M1251/M1252

CLASSIC DNA MARKER of GDSBIO 

Product Name

500bp Ladder

Cat. No./Spec.

M1251/50 μg, M1252/50 μg×5

Description

500bp Ladder is an ideal DNA Marker for determining the size of double-stranded DNA (dsDNA) from 500 to 5,000 base pairs (bp). The ladder consists of 10 linear dsDNA fragments. The 2,000bp fragment is present at increased intensity to allow easy identification. All fragments are precisely quantified and mixed during the production. This ladder is pre-mixed with loading dye and is ready to use.

Application

• agarose gel electrophoresis, AGE

• Non-denaturing polyacrylamide gel electrophoresis (PAGE)

• pulsed field gel electrophoresis, PFGE

Features

• Clear and bright bands for accurate molecular weight determination

• Stable quality allows for use at room temperature

• Multiple ranges available with 23 classic series and 18 LD series options to suit different gel dyes

• Two Marker series are suitable for different types of gel dyes, and the strips are not easily deformed

Recommended Amount

2-5 µl/lane

Recommended Electrophoresis Condition

5 µl/lane, 8 cm 1% Agarose Gel, 1×TAE, 7 V/cm, 45min.

Bands (bp)

500、1,000、1,500、2,000、2,500、3,000、3,500、4,000、4,500、5,000.

Concentration

Total concentration, 92 ng/µl

Typical bands, 100 ng/5µl

Other bands, 40 ng/5µl

Storage Condition

Store unopened at room temperature or 4 ̊C for 2 years.

Components