V5011 One-step Probe RT-qPCR Kit V3

Products & Applications > 1-step RT-qPCR (probe-based) > V5011 One-step Probe RT-qPCR Kit V3

V5011 One-step Probe RT-qPCR Kit V3

Price： $351.00~1228.00

Cat. No./Sp：

V5011(200 rxns) V5011-2(1000 rxns)

Product details

Product Introduction

The One-step Probe RT-qPCR Kit V3 is designed for quantitative PCR detection using RNA as a template, such as viral RNA. Using gene-specific primers (GSP), reverse transcription and qPCR reactions are performed in a single tube, requiring no additional tube opening/pipetting, significantly increasing detection throughput and reducing the risk of contamination. This Kit introduced dUTP/UDG system. Heat-labile UDG can rapidly degrade the pollutants containing U at room temperature. When reverse transcription is performed at 55°C, heat-labile UDG will be inactivated rapidly without affecting the efficiency and sensitivity of RT-qPCR. Integrating the superior performance of Reverse Transcriptase and hot-start Taq DNA Polymerase, with the optimized buffer system, the detection sensitivity of One-step Probe RT-qPCR Kit can reach 0.1 pg total RNA or <10 copies of RNA template. The kit is available as a convenient Master Mix. 5 × One-step U+ Mix contains an optimized buffer system and dNTP/dUTP Mix, which is suitable for high-specificity, low template concentration and multiple detection systems with fluorescent labeled probes such as TaqMan®. This product is perfectly compatible with common quantitative PCR instruments, such as ABI, Roche, Bio-Rad, etc.

Selection Guide of One-Step Kit (probe based):

Cat. No.	V5001/V5002	V5005/V5006	V5005L/V5006L	V5007/V5008	V5007L/V5008L	V5009/V5010	V5011
Product Name	One-step Probe RT-qPCR Kit	DSPath^TM 4X One-Step Multiplex Master Mix	DSPath^TM 4X One-Step Multiplex Master Mix (Lyophilized)	2X One Step Prime RT-qPCR Mix	2X One Step Prime RT-qPCR Mix (Lyophilized)	One-step Probe RT-qPCR Kit V2	One-step Probe RT-qPCR Kit V3
Mixture Format	Enzyme Mix+2X Buffer Mix	4X All-in Mix	4X All-in Lyophilized Powder	2X All-in Mix	2X All-in Lyophilized Powder	Enzyme Mix+2X Buffer Mix	Enzyme Mix+5X Buffer Mix
Mode/Time	S: ~120 min	F: ~60 min S: ~120 min	F: ~60 min S: ~120 min	F: ~60 min S: ~120 min	F: ~60 min S: ~120 min	F: ~60 min S: ~80 min	F: ~45 min S: ~80 min
RT TEMP	50~55°C	48~55°C	48~55°C	48~55°C	48~55°C	50~55°C	50~55°C
PCR Enzyme	Antibody-modified hotstart Taq	Antibody-modified hotstart Taq	Antibody-modified hotstart Taq	Antibody-modified hotstart Taq	Antibody-modified hotstart Taq	Antibody-modified hotstart Taq	Antibody-modified hotstart Taq
Heat-labile UDG	+	+	+	+	+	+	+
Sensitivity	Very high	Very high	Very high	Very high	Very high	Ultra high	Ultra high

S: standard mode; F: fast mode

V5005, V5009, and V5011 are recommended

Application

• Probe gene expression analysis

• Probe Low-copy gene detection

• Probe microarray validation

• Probe gene knockdown validation

Features

• Fast start-up, 30s hot start

• Reverse transcription at 55 °C to accommodate complex RNA templates

• Contains heat-labile UDG to reduce false-positive contamination

• High sensitivity for detection of low-abundance genes

• High stability, unchanged performance at 4°C and 37°C for one week

• The reaction system can be formulated up to 30 minutes in advance without changing performance

• Saves time by supporting fast program

Experimental Cases

Using V5011 for nucleic acid detection of coronavirus can accurately detect the E gene, N1 gene, N2 gene, and ORF1ab sequence

Components

Component	V5011 (200 rxns)	V5011-2 (1000 rxns)
5X One-step U⁺ Mix	800 μl	1 ml × 4
One-step U⁺ Enzyme Mix V3	200 μl	1 ml
RNase-free ddH2O	1 ml	15 ml