P2091b SYBR® Green qPCR Mix (High ROX+)

Product Introduction

SYBR® Green qPCR Mix (High ROX+) is designed for high-performance, high-throughput real-time PCR. The kit contains Taq DNA Polymerase engineered through a process of molecular evolution. The result is a unique polymerase that specifically designed for qPCR using SYBR® Green I chemistry dye.

2X SYBR® Green qPCR Mix (High ROX+) is a convenient premix of the components (except primers and DNA template) that necessary to perform real-time polymerase chain reaction (PCR) using SYBR® Green I dye with enhanced sensitivity and specificity. The SYBR® Green I dye binds to double-stranded DNA (dsDNA), thus providing a fluorescent signal that reflects the amount of dsDNA products generated during PCR.

Application

• Gene expression analysis

• Low-copy gene detection

• Microarray validation

• Gene knockdown validation

Features

• Compatible with many Real-time systems which requires high concentrtion of ROX reference dye to calibration

• Hot-start technology brings high specificity and reproducible amplification

Experimental Cases

After the hot start upgrade, the amplification performance of SYBR® Green qPCR MIX is further improved, and the amplification efficiency and specificity are perfectly balanced.

Components

Storage Condition

Store at -20°C and protected from light