Product Introduction
2× Long Taq Mix is a premixed, ready-to-use solution containing hotstart Taq DNA Polymerase with antibody modification, dNTPs, Mg2+ and Reaction Buffer at optimal concentrations for efficient amplification of DNA templates by PCR. To prepare the final PCR, only need to add primers and template DNA. This premixed formulation saves time and reduces contamination due to the fewer pipetting steps required for PCR set-up. It also contributes to higher sensitivity by adding enhancer.
Application
• PCR amplification of complex template DNA
• PCR amplification of long DNA sequences
• DNA sequencing
• PCR for cloning
Features
• Convenient: only primers and template are needed to add when preparing PCR system
• Longer fragment: amplify long templates as long as 20 kb
• High efficiency: saving your time by simplifying the process
• Reproducible: lower contamination and pipetting error risk
• Amplification of complex template (GC-rich or repetitive sequence)
Experimental Cases
After the upgrade, Long Taq Mix significantly improves PCR amplification ability.
Components
Component | P2061 | P2062 |
2× Long Taq Mix* | 1 ml | 1 ml × 3 |
PCR Enhancer | 0.5 ml | 0.5 ml × 3 |
Nuclease-free water | 1 ml | 1 ml × 3 |
* Available in two versions: bromophenol blue plus and bromophenol blue free (suggested).
Storage Condition
Store at -20°C