CSM Taq Polymerase
For research use only
Cat. No./Spec.
Cat. No | P1231 | P1232 | P1233 | P1234 |
Spec. | 250U | 1,000U | 5,000U | 50,000U |
Description
CSM Taq Polymerase of GDSBio is a Cold Sensitive Mutant enzyme with low activity at room temperature and reactivation above 60°C. Provides excellent specificity, sensitivity, and yield. Amplify fragments up to 5 kb in length (simple template). The elongation rate is 2 min/kb (70-75°C, up to 20 s/kb for simple templates). It has 5’-3’ polymerase activity, but no 3’-5’ exonuclease activity. The products of CSM Taq Polymerase have overhanged dA at 3’-end.
Components
Component | P1231 | P1232 | P1233 | P1234 |
CSM Taq Polymerase | 50 μl | 200 μl | 1 ml | 1 ml × 10 |
10× Hotstart Buffer(Mg2+ Plus) | 1.25 ml | 1.25 ml × 2 | 1.25 ml × 10 | 50 ml × 10 |
Application
high specificity PCR
Features
1. Mutant cold-sensitive heat-activated enzyme, with low activity at room temperature.
2. Activity is restored above 60°C, effectively inhibiting non-specific amplification and primer dimer formation.
3. High sensitivity.
4. The product has a 3'-dA overhang.