Product Introduction
2× Optimus Hotstart Taq Mix is a premixed, ready-to-use solution containing Hotstart Taq DNA Polymerase, dNTPs, Mg2+ and Reaction Buffer at optimal concentrations for efficient amplification of DNA templates by PCR. To prepare the final PCR, only need to add primers and template DNA. This premixed formulation saves time and reduces contamination due to the fewer pipetting steps required for PCR set-up. The mix retains all features of Hotstart Taq DNA Polymerase.
Application
•High-specificity amplification & multiplex PCR: hot-start polymerase with antibody modification
• Thermostable: half-life over 40 min at 95°C incubation
• Generates 3'-dA overhangs PCR products
Features
• High specificity
• hotstart with antibody modification
• Elongation speed: 1kb/min
• generates 3'-dA overhangs
Experimental Cases
After the upgrade, Optimus Hotstart Taq Mix (P2041) further improves the amplification efficiency on the basis of ensuring high specificity.
Components
Component | P2041 | P2042 | P2043 | P2044 |
2× Optimus Hotstart Taq Mix* | 1 ml | 1 ml × 5 | 100 ml | 500ml |
Nuclease-free water | 1 ml | 1 ml × 5 | - | - |
* Available in two versions: bromophenol blue plus and bromophenol blue free (suggested).
Storage Condition
Store at -20°C