Super HIFI DNA Polymerase P1251 P1252

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Super HIFI DNA Polymerase P1251 P1252

Price： $108.00~430.00

Cat. No.：

P1251/100 U P1252/500 U

Product details

Super HIFI DNA Polymerase

For research use only

Cat. No./Spec.

Cat. No	P1251	P1252
Spec.	100U	500U

Description

Super HIFI DNA Polymerase is a high-fidelity DNA polymerase. Its fidelity is approximately 50 times higher than that of regular Taq DNA polymerase, making it suitable for cloning and other applications that require high fidelity. Compared to other DNA polymerases, Super HIFI DNA Polymerase is robust, with short protocol time, tolerance to PCR inhibitors, and high yields with fewer enzymes. Amplified fragments can be up to 7.5 kb genome and 20 kb lambda DNA in length. The enzyme has 5'→3' polymerase activity and 3'→5' exonuclease activity, and the amplification product has blunt ends.

Applications

gene clone; high fidelity PCR

Features

50X high fidelity of Taq

Tolerant to PCR inhibitors

strong amplification capability for long fragments

Components

Component	P1251	P1252
Super HIFI DNA Polymerase（2U/μl）	50 μl	250 μl
5X Super HF Buffer ^[¹^]	1.5 ml × 2	1.5 ml × 6
5X Super GC Buffer ^[¹^]	1.5 ml	1.5 ml × 2
MgCl₂ (50 mM)	1.5 ml	1.5 ml × 2
DMSO	500 μl	500 μl

[1] Both 5X Super HF Buffer and 5X Super GC Buffer provide a final concentration of 1.5 mM MgCl₂.

Unit Definition

One unit is defined as the amount of enzyme required to incorporate 10 nmol of deoxynucleotide into acid-insoluble species over 30 minutes at 72°C using activated salmon sperm DNA as template/primer.

Storage

Store at -20°C for 2 years.