Product Introduction
2× Taq Mix Ⅱ is an upgraded product of Taq Mix, with better sensitivity and specificity. It’s a premixed, ready-to-use solution containing Taq DNA Polymerase, dNTPs, Mg2+ and Reaction Buffer at optimal concentrations for efficient amplification of DNA templates by PCR. To prepare the final PCR, only need to add primers and template DNA. This premixed formulation saves time and reduces contamination due to the fewer pipetting steps required for PCR set-up. The mix retains all features of Taq DNA Polymerase.
Application
• High throughput PCR
• Routine PCR with high reproducibility
• Generation of PCR products for TA cloning
Features
• Convenient: only primers and template DNA are added when preparing PCR system
• High yields of PCR products with minimal optimization
• High efficiency: saving your time by simplifying the process
• Reproducible: lower contamination and pipetting error risk
Experimental Cases
The Taq Mix was able to maintain stable performance for 5 days at 37°C and successfully amplify 1000bp fragments.
Components
Component | P2011b | P2012b |
2× Taq Mix II * | 1 ml | 1 ml × 5 |
Nuclease-free water | 1 ml | 1 ml × 5 |
* Available in two versions: bromophenol blue plus and bromophenol blue free (suggested).
Storage Condition
Store at -20°C