P2021 Pfu Mix

Product Introduction

2× Pfu Mix is a premixed, ready-to-use solution containing hotstart Pfu DNA Polymerase with antibody modification, dNTPs, Mg2+ and Reaction Buffer at optimal concentrations for efficient amplification of DNA templates by PCR. To prepare the final PCR, only need to add primers and template DNA.  This premixed formulation saves time and reduces contamination due to the fewer pipetting steps required for PCR set-up. It also contributes to higher sensitivity by adding enhancer.

Application

• High fidelity PCR

• Routine PCR with high reproducibility

• Site-directed mutagenesis

• Generation of PCR products for TA cloning

Features

• 10X high fidelity of Taq

• Convenient: only primers and template DNA are added when preparing PCR system

• High yields of PCR products with minimal optimization

• Fast: saves time due to reduced number of pipetting steps.

• Reproducible: lower contamination and pipetting error risk

Experimental Cases

After the upgrade, Pfu Mix significantly improves PCR amplification efficiency while maintaining fidelity.

Components

* Available in two versions: bromophenol blue plus and bromophenol blue free (suggested).

Storage Condition

Store at -20°C