P1021 Pfu DNA Polymerase

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P1021 Pfu DNA Polymerase

Price： $13.20~56.00

Cat. No./Sp：

P1021(250 U) P1022(250 U (dNTPs+)) P1023(1,000 U) P1024(1,000 U (dNTPs+))

Product details

Product Introduction

Pfu DNA polymerase, derived from the hyperthermophilic archae Pyrococcus furiosus, has superior thermostability and proofreading properties compared to other thermostable polymerase. Its molecular weight is 90 kDa. It can amplify DNA target up to 2 kb（simple template）. The elongation velocity is 1kb/min(70~75°C). Pfu DNA polymerase possesses 3' to 5' exonuclease proofreading activity that enables the polymerase to correct nucleotide-misincorporation errors. This means that Pfu DNA polymerase-generated PCR fragments will have fewer errors than Taq-generated PCR inserts. Using Pfu DNA polymerase in your PCR reactions results in blunt-ended PCR products, which are ideal for cloning into blunt-ended vectors. Pfu DNA polymerase is superior for techniques that require high-fidelity DNA synthesis.

Application

• High-fidelity PCR and primer-extension reactions

• High fidelity PCR for cloning into blunt-ended vectors

• Site-directed mutagenesis

Features

• 10X high fidelity of Taq

• Elongation speed: 1kb/min

• generates blunt ends

Components

Component	P1021 (250U)	P1022 (250U)	P1023 (1000U)	P1024 (1000U)
Pfu DNA Polymerase (2.5U/μl)*	100 μl	100 μl	400 μl	400 μl
10× Pfu Buffer (Mg2+ Plus)**	1.25 ml	1.25 ml	1.25 ml × 2	1.25 ml × 2
dNTPs (2.5mM each)	-	1 ml	-	1 ml × 2
6× Loading Buffer	1 ml	1 ml	1 ml	1 ml