Product Introduction
HS Taq DNA Polymerase is a thermostable recombinant DNA polymerase derived from thermophilic bacterium Thermus aquaticus. Its molecular weight is 94 kDa. HS Taq DNA Polymerase can amplify DNA target up to 5 kb. The elongation velocity is 2kb/min. It has 5' to 3' polymerase activity but lacks of 3' to 5' exonuclease activity that results in a 3'-dA overhangs PCR product. All components of the HS PCR Buffer are at optimal concentration for efficient amplification. It contributes to highly specific incorporation of primer and template.
Application
• PCR amplification of DNA fragments as long as 5 kb
• DNA labeling
• DNA sequencing
• PCR for cloning
Features
High specificity (not hotstart), 2kb/min, generates 3'-dA overhangs
Components
Component | P1081 (250U) | P1082 (500U) | P1083 (1000U) | P1084 (18000U) |
HS Taq DNA Polymerase (5U/μl) | 50 μl | 100 μl | 200 μl | 100 μl × 36 |
10× HS PCR Buffer (Mg2+ Plus) * | 1.25 ml | 1.25 ml | 1.25 ml × 2 | 1.25 ml × 36 |
6× Loading Buffer | 1 ml | 1 ml | 1 ml | - |
* Mg2+ Free version is also available.
Storage Condition
Store at -20°C