NGS Multiplex PCR Master MixIII NM3001/NM3002/NM3003

Product Introduction

NGS Multiplex PCR Master MixIII is a PCR Master Mix designed for NGS unbiased target amplification. By optimizing the enzyme and buffer, this product significantly improves the success rate and consistency of amplification efficiency for GC/AT rich amplification. Primers with different GC content can use a uniform annealing temperature of 55°C to achieve unbiased PCR amplification, up to 10,000x multiplex PCR, which can be used for viral, microbial, and tumor/hereditary DNA target NGS sequencing. It is also suitable for long-fragment multiplex PCR, with the longest fragment reaching up to 20kb, meeting the long-fragment multiple amplification needs for third-generation sequencing.

Application

This product is suitable for PCR amplification of NGS libraries.

Features

Stale amplification efficiency.

strong amplification with complex template.

uniform annealing temperature of 55°C

Supports 10,000x multiplex PCR.

Tolerates various sample inhibitors.

Components

Storage Condition

Store unopened at –15°C to –25°C until the expiration date on the label. After opening, the master mix may be stored at –15°C to –25°C until the expiration date on the label, or at 4°C for up to 30 days.