Super Probe qPCR Mix Plus U
For research use only
Cat. No./Spec.
Cat. No. | P2711 | P2712 | P2713 | P2714 |
25-μl rxn Nos | 80 rxns | 400 rxns | 4000 rxns | 8000 rxns |
Components
Component | P2711 | P2712 | P2713 | P2714 |
2× Super Probe qPCR Mix Plus U | 1 ml | 1 ml × 5 | 50 ml | 100 ml |
Nuclease-free Water | 1 ml | 1 ml × 5 | - | - |
Storage
This reagent can be stored for 2 months at 4°C. For longer storage, it should be kept at -20°C.
Description
Super Probe qPCR Mix Plus U is a 2× concentrated premix for real-time quantitative PCR with probe method. In use, just add the DNA template, primer and probe to react. This product contains a Hotstart Taq DNA polymerase modified with antibody technology, combined with Dongsheng's specially formulated Super PCR Buffer, which enables multiplex qPCR and is tolerant to PCR inhibitors such as SDS, guanidine salts, and heparin. This reagent introduced dUTP/UDG anti-contamination system, which can remove PCR products containing dUTP before PCR reaction, effectively avoid the influence of cross contamination of amplification products on quantification. This product is convenient for use in conjunction with TaqMan and primers to achieve a fully premixed Master Mix molecular diagnostic kit. This product can be used with TaqMan and other fluorescent probes, and is perfectly compatible with common quantitative PCR instruments, such as ABI, Roche, Bio-rad, etc.
The reaction system of this product can be prepared at room temperature without an ice box. The prepared PCR reaction system can be placed at room temperature for 24 hours and the amplification efficiency remains unchanged.
Application
• Probe gene expression analysis
• Probe Low-copy gene detection
• multiplex probe qPCR
• IVD diagnostic kit research
Features
• Ultra-high sensitivity for multiplex probe qPCR
• Hot-start technology brings high specificity and reproducible amplification
• dUTP/UDG system, effectively prevent PCR product contamination
• Compatible with many real-time systems