Product Introduction
qPCR Hotstart Taq DNA Polymerase is a hot-start Taq DNA polymerase with antibody modification, and the activity is wholly inhibited at room temperature, which results in less non-specific amplifications. Depending on high temperature to activate the enzyme activity, the reaction has very high specificity and sensitivity. qPCR Hotstart Taq DNA Polymerase adopts advanced production technology, does not introduce contamination of animal-derived DNA. The enzyme has stronger stability, and incomparable advantages over antibody-modified hot-start Taq DNA polymerase. The pre-denaturation time is reduced to 3 minutes, and the amplification efficiency is higher than that of most chemical-modified hot-start DNA polymerase. The elongation rate is 1kb/min. It is a special polymerase for real-time quantitative PCR (qPCR), both dye method and probe method.
Application
• High-specificity amplification & multiplex PCR
• High fidelity PCR
• Gene expression analysis
• Complex template low-copy gene amplification
Features
• hot-start polymerase with antibody modification
• Thermostable: half-life over 40 min at 95°C incubation
• Generates 3'-dA overhangs PCR products
Components
Component | P1101 (250U) | P1102 (1000U) | P1103 (3000U) | P1104 (18000) |
qPCR Hotstart Taq DNA Polymerase (5 U/μl) | 50 μl | 200 μl | 200 μl × 3 | 100 μl × 36 |
10× qPCR Buffer | 1.25 ml | 1.25 ml × 2 | 1.25 ml × 6 | 1.25 ml × 36 |
Storage Condition
Store at -20°C