Product Introduction
Optimus Hotstart Taq DNA Polymerase is a hot-start polymerase with antibody modification, which brings higher specificity by reducing non-specific products as the enzyme activity is temperature-dependent and is inhibited at room temperature. The amplification length and speed can reach to 5 kb (simple template) and 1min/kb (simple template up to 20s/kb) separately. Hotstart Taq has 5’-3’ polymerase activity, but no 3’-5’ exonuclease activity. The product of Hotstart Taq has overhanged dA at 3’-end. Optimus Hotstart Taq DNA Polymerase has zero animal source pollution by being produced with advanced antibody modification. Its efficiency is higher than most antibody-modified polymerase and the initial-denaturation time can be reduced to 3 minutes.
Optimus Hotstart Taq DNA polymerase is an innovative and useful product.
Application
• High-specificity amplification & multiplex PCR: hot-start polymerase with antibody modification
• Thermostable: half-life over 40 min at 95°C incubation
• Generates 3'-dA overhangs PCR products
Features
• High specificity
• hotstart with antibody modification
• Elongation speed: 1kb/min
• generates 3'-dA overhangs
Components
Component | P1041 (250U) | P1042 (1000U) | P1043 (3000U) | P1044 (18000U) |
Optimus Hotstart Taq DNA Polymerase (5U/ul) | 50 μl | 200 μl | 200 μl × 3 | 100 μl × 36 |
10× Hotstart Buffer (Mg2+ Plus)* | 1.25 ml | 1.25 ml × 2 | 1.25 ml × 6 | 1.25 ml × 36 |
6× Loading Buffer | 1 ml | 1 ml | 1 ml | - |
* Mg2+ Free version is also available.
Storage Condition
Store at -20°C