Product Introduction
Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) uses single-stranded RNA or DNA in the presence of a primer to synthesize a complementary DNA strand. This enzyme is isolated from E. coli expressing a portion of the pol gene of M-MLV on a plasmid. The enzyme is used to synthesize first-strand cDNA up to 5 kb.
Concentration: 200U/μl
Application
1st strand cDNA synthesis,especially for two-step RT-PCR/qPCR
Features
The optimum activity temperature is 37-42°C
Components
Component | R1041 (5000U) | R1042 (10000U) |
M-MLV | 25 μl | 50 μl |
5X first-strand buffer | 100 μl | 200 μl |
Storage Condition
Store at -20°C