Product Introduction
#K003 UDI UMI Adapter has a 6 nt random complementary sequence, allowing for the addition of 6 nt UMI (Unique Molecular Identifiers) at both ends of the library after adapters ligation. The UMI allows multiplex analysis for multiple samples simultaneously, reducing the detection rate of false positive variants and improving the sensitivity of detection for low frequency mutations. Each pre-mixed UDI Primer contains a unique pair of one 8nt i5 index and one 8nt i7 index primer, which can be independently double-checked by library amplification to meet the high-throughput sequencing mixing requirements and greatly reduce the sample index misassignment.
Application
This product is a special adapter primer kit for #K001 Fast DNA Library Prep Kit and #K004 Fast DNA Library Plus Prep Kit, which is applicable for Illumina platform.
Features
1 long adapter
2 Avoid false positives
3 Avoid label jumping (mismatch)
Experimental Cases
无
Components
Component | K003-A (96 rxns) | K003-B (96 rxns) | K003-C (96 rxns) | K003-D (96 rxns) |
UMI Adapter | 480μl | 480μl | 480μl | 480μl |
UDI Primer (UDI 001 - UDI 024) | 20μl/each | / | / | / |
UDI Primer (UDI 001 - UDI 024) | / | 20μl/each | / | / |
UDI Primer (UDI 001 - UDI 024) | / | / | 20μl/each | / |
UDI Primer (UDI 001 - UDI 024) | / | / | / | 20μl/each |
Storage Condition
All reagents should be stored at -20°C.