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Klenow Fragment(3´→5´ exo-) K010-A K010-B K010-C

Products & Applications > NGS Library Preparation Reagents > Klenow Fragment(3´→5´ exo-) K010-A K010-B K010-C

Klenow Fragment(3´→5´ exo-) K010-A K010-B K010-C

Price: $17.00~71.00
K010-A(100 U) K010-B(200 U) K010-C(1,000 U)

Product Introduction

Klenow Fragment (3´→ 5´ exo-) is an N-terminal truncation of DNA Polymerase I which retains polymerase activity, but has lost the 5´→ 3´ exonuclease activity and has mutations which abolish the 3´→ 5´ exonuclease activity.

Application

1.Generates probes using random primers

2.Random priming labeling

3.dA-tailing at 3' end

4.Second strand cDNA synthesis

Features

Moderate strand displacement activity

Experimental Cases

Components

Component

K010-A

(100U)

K010-B

(200U)

K010-C

(1,000U)

Klenow Fragment3´→5´ exo-(5U/µL)

20 µL

40 µL

200 µL

10X Klenow Buffer

1.25 mL

1.25 mL

1.25 mL

Storage Condition

All reagents should be stored at -20°C.


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