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V5011 One-step Probe RT-qPCR Kit V3

Products & Applications > Molecular Research Reagents > V5011 One-step Probe RT-qPCR Kit V3

V5011 One-step Probe RT-qPCR Kit V3

Price: $351.00~1228.00
V5011(200 rxns) V5011-2(1000 rxns)

Product Introduction

The One-step Probe RT-qPCR Kit V3 is designed for quantitative PCR detection using RNA as a template, such as viral RNA. Using gene-specific primers (GSP), reverse transcription and qPCR reactions are performed in a single tube, requiring no additional tube opening/pipetting, significantly increasing detection throughput and reducing the risk of contamination. This Kit introduced dUTP/UDG system. Heat-labile UDG can rapidly degrade the pollutants containing U at room temperature. When reverse transcription is performed at 55°C, heat-labile UDG will be inactivated rapidly without affecting the efficiency and sensitivity of RT-qPCR. Integrating the superior performance of Reverse Transcriptase and hot-start Taq DNA Polymerase, with the optimized buffer system, the detection sensitivity of One-step Probe RT-qPCR Kit can reach 0.1 pg total RNA or <10 copies of RNA template. The kit is available as a convenient Master Mix. 5 × One-step U+ Mix contains an optimized buffer system and dNTP/dUTP Mix, which is suitable for high-specificity, low template concentration and multiple detection systems with fluorescent labeled probes such as TaqMan®. This product is perfectly compatible with common quantitative PCR instruments, such as ABI, Roche, Bio-Rad, etc.

 

Selection Guide of One-Step Kit (probe based):

Cat. No.


V5001/V5002

V5005/V5006

V5005L/V5006L

V5007/V5008

V5007L/V5008L

V5009/V5010

V5011

Product Name


One-step Probe RT-qPCR Kit

DSPathTM 4X One-Step Multiplex Master Mix

DSPathTM 4X One-Step Multiplex Master Mix (Lyophilized)

2X One Step Prime RT-qPCR Mix

2X One Step Prime RT-qPCR Mix (Lyophilized)

One-step Probe RT-qPCR Kit V2

One-step Probe RT-qPCR Kit V3

Mixture Format


Enzyme Mix+2X Buffer Mix

4X All-in Mix

4X All-in Lyophilized Powder

2X All-in Mix

2X All-in Lyophilized Powder

Enzyme Mix+2X Buffer Mix

Enzyme Mix+5X Buffer Mix

Mode/Time


S: ~120 min

F: ~60 min S: ~120 min

F: ~60 min S: ~120 min

F: ~60 min S: ~120 min

F: ~60 min S: ~120 min

F: ~60 min S: ~80 min

F: ~45 min S: ~80 min

RT TEMP


50~55°C

48~55°C

48~55°C

48~55°C

48~55°C

50~55°C

50~55°C

PCR Enzyme


Antibody-modified hotstart Taq

Antibody-modified hotstart Taq

Antibody-modified hotstart Taq

Antibody-modified hotstart Taq

Antibody-modified hotstart Taq

Antibody-modified hotstart Taq

Antibody-modified hotstart Taq

Heat-labile UDG


+

+

+

+

+

+

+

Sensitivity


Very high

Very high

Very high

Very high

Very high

Ultra high

Ultra high

S: standard mode; F: fast mode

V5005, V5009, and V5011 are recommended

 

Application

Probe gene expression analysis

Probe Low-copy gene detection

Probe microarray validation

Probe gene knockdown validation

Features

Fast start-up, 30s hot start

Reverse transcription at 55 °C to accommodate complex RNA templates

Contains heat-labile UDG to reduce false-positive contamination

High sensitivity for detection of low-abundance genes

High stability, unchanged performance at 4°C and 37°C for one week

The reaction system can be formulated up to 30 minutes in advance without changing performance

Saves time by supporting fast program

Experimental Cases

Using V5011 for nucleic acid detection of coronavirus can accurately detect the E gene, N1 gene, N2 gene, and ORF1ab sequence

1703152907354.jpg

Components

Component

V5011

(200 rxns)

V5011-2

(1000 rxns)

5X One-step U+ Mix

800 μl  

1 ml × 4 

One-step U+ Enzyme Mix V3

200 μl  

1 ml

RNase-free ddH2O

1 ml

15 ml

 

Storage Condition

Store at -20°C


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