Product Introduction
2× Plus Mix is a premixed, ready-to-use solution containing hotstart Taq Plus DNA Polymerase with antibody modification, dNTPs, Mg2+ and Reaction Buffer at optimal concentrations for efficient amplification of DNA templates by PCR. To prepare the final PCR, only need to add primers and template DNA. This premixed formulation saves time and reduces contamination due to the fewer pipetting steps required for PCR set-up. It also contributes to higher sensitivity by adding enhancer.
Application
• Long PCR with high fidelity
• High reproducible PCR for complex templates
• High throughput PCR for complex templates
• Generation of PCR products for TA cloning
Features
• Convenient: only primers and template DNA are added when preparing PCR system
• High efficiency: saving your time by simplifying the process
• Reproducible: lower contamination and pipetting error risk
Experimental Cases
After the upgrade, Plus Mix significantly improves PCR specificity while maintaining high amplification efficiency.
Components
Component | P2031 | P2032 |
2× Plus Mix* | 1 ml | 1 ml × 5 |
Nuclease-free water | 1 ml | 1 ml × 5 |
* Available in two versions: bromophenol blue plus and bromophenol blue free (suggested).
Storage Condition
Store at -20°C