P1031 Plus DNA Polymerase

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P1031 Plus DNA Polymerase

Price： $11.00~44.10

Cat. No./Sp：

P1031(250 U) P1032(250 U (dNTPs+)) P1033(1,000 U) P1034(1,000 U (dNTPs+))

Product details

Product Introduction

Taq Plus DNA Polymerase is a mixture of Taq and Pfu polymerase, blends the processivity of taq with the high fidelity of Pfu. The two enzymes act synergistically during PCR to generate more accurate and longer PCR products with greater yields compared to Taq DNA Polymerase alone. The elongation rate is 3kb/min. It can amplify DNA target up to 20 kb (simple template). And it is suitable as a direct replacement for ordinary Taq Polymerase in most applications. PCR products amplified by Taq Plus are mixture of blunt-ends and 3'dA-overhangs. The error rate of this PCR amplification is 1.0×10-5 per nucleotide per cycle.

Application

• Amplification of long template up to 20 kb

• Amplification of complex template

• High fidelity PCR

Features

High efficiency, 3kb/min, generates 3'-dA overhangs and blunt ends

Components

Component	P1031 (250U)	P1032 (250U)	P1033 (1000U)	P1034 (1000U)
Taq Plus DNA Polymerase (2.5U/μl)*	100 μl	100 μl	400 μl	400 μl
10× PCR Buffer (Mg2+ Plus)*	1.25 ml	1.25 ml	1.25 ml × 2	1.25 ml × 2
dNTPs (2.5mM each)	-	1 ml	-	1 ml × 2
6× Loading Buffer	1 ml	1 ml	1 ml	1 ml