Product Introduction
Bst DNA Polymerase, Exonuclease Minus is derived from Bacillus stearothermophilus DNA Polymerase I. This enzyme possesses 5'-3' polymerase activity, strand displacement activity, and dUTP tolerance, while the 5'-3' exonuclease activity has been removed. This product is suitable for contamination-resistant DNA isothermal amplification reactions, such as LAMP (loop-mediated isothermal amplification), SDA (cross-priming amplification), and other isothermal amplification techniques.
Application
LAMP, CPA, RCA, and other isothermal amplification reactions.
Features
• Strong chain displacement activity
• Excellent amplification performance
• High specificity
Components
Component | P1111 | P1112 | P1113 | P1114 |
Bst DNA Polymerase, Exonuclease Minus | 25 μl | 50 μl | 200 μl | 1 ml |
10X DNA Polymerase Buffer B | 125 μl | 250 μl | 1 ml | 1 ml × 3 |
MgSO4 (100 mM) | 125 μl | 250 μl | 1 ml | 1 ml × 3 |
Storage Condition
Store at -20°C