P2101 power Green qPCR Mix (NO ROX)

Product Introduction

Power Green qPCR Mix is a ready-to-use, 2X concentrated mix that contains all the reagents (except template and primers) needed for the real-time qPCR in the SYBR Green I detection format. This product is compatible with most manufacturers' real-time fluorescent quantitative PCR instruments such as Applied Biosystems, Roche, Bio-Rad, Eppendorf, Corbett and so on.

The Hotstart Taq DNA Polymerase in the mix is modified by antibody. When the temperature below 45°C, the activity of the modified polymerase will be strongly suppressed, only when the temperature reached 72°C, it can be activated. This technology helps to reduce non-specific amplification and primer dimer effectively. 

Application

• Gene expression analysis

• Low-copy gene detection

• Microarray validation

• Gene knockdown validation

Features

• Compatible with many Real-time systems which not require ROX reference dye

• Exceptional specificity with hot-start mechanism

• Tight reproducibility in Ct values over a broad dynamic range

• Universal instrument compatibility

Experimental Cases

After the hot start upgrade, Amplification efficiency of Power Green qPCR Mix is further improved while maintaining high specificity.

Components

Storage Condition

Store at -20°C and protected from light