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P2101 power Green qPCR Mix (NO ROX)

Products & Applications > Molecular Research Reagents > P2101 power Green qPCR Mix (NO ROX)

P2101 power Green qPCR Mix (NO ROX)

Price: $38.00~150.00
P2101(1 mL) P2102(1 mL×5)

Product Introduction

Power Green qPCR Mix is a ready-to-use, 2X concentrated mix that contains all the reagents (except template and primers) needed for the real-time qPCR in the SYBR Green I detection format. This product is compatible with most manufacturers' real-time fluorescent quantitative PCR instruments such as Applied Biosystems, Roche, Bio-Rad, Eppendorf, Corbett and so on.

The Hotstart Taq DNA Polymerase in the mix is modified by antibody. When the temperature below 45°C, the activity of the modified polymerase will be strongly suppressed, only when the temperature reached 72°C, it can be activated. This technology helps to reduce non-specific amplification and primer dimer effectively. 

Application

• Gene expression analysis

• Low-copy gene detection

• Microarray validation

• Gene knockdown validation

Features

• Compatible with many Real-time systems which not require ROX reference dye

• Exceptional specificity with hot-start mechanism

• Tight reproducibility in Ct values over a broad dynamic range

• Universal instrument compatibility

Experimental Cases

After the hot start upgrade, Amplification efficiency of Power Green qPCR Mix is further improved while maintaining high specificity.

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Components

Component

P2101

P2102

2X Power Green qPCR Mix

1 ml

1 ml × 5

Nuclease-free Water

1 ml

1 ml × 5

 

Storage Condition

Store at -20°C and protected from light


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