V5005L DSPath 4X One-Step Multiplex Master Mix (Lyophilized)

Product Introduction

The DSPath 4X One-Step Multiplex Master Mix is used to perform one-step multiplex real-time PCR applications with any gene-specific primer and probe sets, and is suitable for both RNA and DNA targets. This master mix is formulated with optimized buffer components to accommodate multiplex amplification of up to four RNA or DNA target sequences in a single reaction. The master mix is supplied at a 4X concentration that allows to input more sample into each reaction, increasing sensitivity even in low-volume reactions. Lyophilized Enzyme Mix is a freeze-dried reagent made of  heat-resistant reverse transcriptase, hot-start Taq DNA polymerase, RNase inhibitor, Heat-labile UDG, dNTPs, etc., which makes the product performance more stable and can be stored for a long time.

Selection Guide of One-Step Kit (probe based):

S: standard mode; F: fast mode

V5005, V5009, and V5011 are recommended

Application

• Probe gene expression analysis

• Probe Low-copy gene detection

• Probe microarray validation

• Probe gene knockdown validation

Features

• Lyophilized powder, transportable and storable at room temperature

• One-step RT-qPCR

• This kit is suitable for fluorescence quantification by probe method

• This kit is compatible with many real-time systems

• Hot-start technology brings high specificity and reproducible amplification

• dUTP/UDG system, effectively prevent PCR product contamination

Experimental Cases

无

Components

Storage Condition

Store at 2~8°C