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V5005L DSPath 4X One-Step Multiplex Master Mix (Lyophilized)

Products & Applications > Molecular Research Reagents > V5005L DSPath 4X One-Step Multiplex Master Mix (Lyophilized)

V5005L DSPath 4X One-Step Multiplex Master Mix (Lyophilized)

Price: $443.00~8615.00
V5005L(200 rxns) V5006L(5000 rxns)

Product Introduction

The DSPath 4X One-Step Multiplex Master Mix is used to perform one-step multiplex real-time PCR applications with any gene-specific primer and probe sets, and is suitable for both RNA and DNA targets. This master mix is formulated with optimized buffer components to accommodate multiplex amplification of up to four RNA or DNA target sequences in a single reaction. The master mix is supplied at a 4X concentration that allows to input more sample into each reaction, increasing sensitivity even in low-volume reactions. Lyophilized Enzyme Mix is a freeze-dried reagent made of  heat-resistant reverse transcriptase, hot-start Taq DNA polymerase, RNase inhibitor, Heat-labile UDG, dNTPs, etc., which makes the product performance more stable and can be stored for a long time.

 

Selection Guide of One-Step Kit (probe based):

Cat. No.


V5001/V5002

V5005/V5006

V5005L/V5006L

V5007/V5008

V5007L/V5008L

V5009/V5010

V5011

Product Name


One-step Probe RT-qPCR Kit

DSPathTM 4X One-Step Multiplex Master Mix

DSPathTM 4X One-Step Multiplex Master Mix (Lyophilized)

2X One Step Prime RT-qPCR Mix

2X One Step Prime RT-qPCR Mix (Lyophilized)

One-step Probe RT-qPCR Kit V2

One-step Probe RT-qPCR Kit V3

Mixture Format


Enzyme Mix+2X Buffer Mix

4X All-in Mix

4X All-in Lyophilized Powder

2X All-in Mix

2X All-in Lyophilized Powder

Enzyme Mix+2X Buffer Mix

Enzyme Mix+5X Buffer Mix

Mode/Time


S: ~120 min

F: ~60 min S: ~120 min

F: ~60 min S: ~120 min

F: ~60 min S: ~120 min

F: ~60 min S: ~120 min

F: ~60 min S: ~80 min

F: ~45 min S: ~80 min

RT TEMP


50~55°C

48~55°C

48~55°C

48~55°C

48~55°C

50~55°C

50~55°C

PCR Enzyme


Antibody-modified hotstart Taq

Antibody-modified hotstart Taq

Antibody-modified hotstart Taq

Antibody-modified hotstart Taq

Antibody-modified hotstart Taq

Antibody-modified hotstart Taq

Antibody-modified hotstart Taq

Heat-labile UDG


+

+

+

+

+

+

+

Sensitivity


Very high

Very high

Very high

Very high

Very high

Ultra high

Ultra high

S: standard mode; F: fast mode

V5005, V5009, and V5011 are recommended

 

Application

Probe gene expression analysis

Probe Low-copy gene detection

Probe microarray validation

Probe gene knockdown validation

Features

Lyophilized powder, transportable and storable at room temperature

One-step RT-qPCR

This kit is suitable for fluorescence quantification by probe method

This kit is compatible with many real-time systems

Hot-start technology brings high specificity and reproducible amplification

dUTP/UDG system, effectively prevent PCR product contamination

Experimental Cases

Components

Component

V5005L

(200 rxns)

V5006L

(5000 rxns)

DSPath Multiplex Enzyme Mix (Lyophilized)

100 rxns × 2

100 rxns × 50 

Enzyme Mix Buffer (4X)  

1 ml × 2

1 ml × 25

 

Storage Condition

Store at 2~8°C


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