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R1051 General RNA Extraction Kit

Products & Applications > Molecular Research Reagents > R1051 General RNA Extraction Kit

R1051 General RNA Extraction Kit

Price: $13.00
R1051(50 preps)

Product Introduction

General RNA Extraction Kit provides a simple method of isolating total RNA from a wide range of sample types and amounts. In general, samples are lysed and then homogenized in the presence of guanidinium isothiocyanate, a chaotropic salt which is capable of protecting the RNA from endogenous RNases. After homogenization, ethanol is added to the sample. The sample is then processed through a spin column containing a clear silica-based membrane where the RNA binds. Any impurities are effectively removed by subsequent washing. The purified total RNA is then eluted in RNase-Free Water and is suitable for use in a variety of downstream applications.  

Application

Purified RNA is free of impurities and enzyme inhibitors, and have an A260/280=1.8-2.0, is suitable for applications such as:

Reverse transcription PCR (RT-PCR)

Real-time quantitative PCR (qPCR)

Northern blotting

Nuclease protection assays

RNA amplification for microarray analysis

cDNA library preparation after poly(A)+ selection

Features

Stable yield

Reliable performance of high-quality purified total RNA in downstream applications

Components

Component

R1051

(50 preps)

Solution RL

60 ml

Wash Buffer RPI

18 ml

Wash Buffer RW

12 ml

DEPC-treated water

10 ml

RNase-free Spin Column

50 each

RNase-free Microcentrifuge Tube

50 each

 

Storage Condition

Solution RL store at 2-8°C, protect from light. Other reagents store at room temperature (RT, 15-25°C). And transport at RT. Kit contents are stable for up to 12 months when stored properly.


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