tel.png Tel: +86 20 31600213    mail.png Sales EMail: order@gdsbio.com      

Zero Background PCR Breakthrough with Enhanced Dual Antibody Modification Hotstart Technology

Column:Product News Time:2024-01-31 Browsing volume: 204

GDSBio has conducted a Hotstart upgrade of dual antibody modification for some of the most widely used PCR products in the field of molecular diagnostics, achieving zero background PCR and improving the accuracy of detection. This technology is GDSBio's patented technology - Enhanced Dual Antibody Modification Hotstart Technology, which uses two monoclonal antibodies to block the active site of DNA polymerase, inhibiting non-specific amplification or primer dimer formation at room temperature and during the PCR reaction process, thereby achieving a background-free amplification.

Technical advantages:

① Enhanced dual antibody modification, with higher specificity than conventional dual antibody modification.

② Zero background products, no non-specific DNA bands, no primer dimers

③ No need for gel cutting, products can be directly recovered for cloning 


Using the upgraded GDSBio #P2111 Super HIFI PCR Master Mix modified with dual antibodies and similar products from manufacturers T, A, and B, we amplified fragments of 400 bp, 600 bp, 800 bp, and 1000 bp using human genomic DNA as a template. The results showed that #P2111 could achieve zero background amplification in four different reactions.

 

Figure 1: Electrophoresis results. M: GDSBio #M1061 100bp Ladder

PCR reagents with enhanced dual antibody modification:

Application

Product Name

Cat. No.

Spec.

Ultra-high fidelity PCR

Super HIFI PCR Master Mix

P2111/P2112/P2113

1 ml/1 ml×10/10 ml×5

ARMS PCR

ARMS PCR Mix

P4011/P4012

1 ml/1 ml×5

KASP

KASP PCR Mix

P4021/P4022

1 ml/1 ml×5

High fidelity PCR

Hotstart Pfu Mix

P2051/P2052

1 ml/1 ml×5

Hotstart PCR

Optimus Hotstart Taq Mix

P2041/P2042

1 ml/1 ml×5

Multiplex PCR

Multiplex PCR Master Mix with UDG

PM2001/PM2002/PM2003

40rnxs/400rnxs/2000rnxs

NGS PCR enrichment

NGS Multiplex PCR Master Mix I

NM1001/NM1002/NM1003

40rnxs/400rnxs/2000rnxs

NGS PCR enrichment

NGS Multiplex PCR Master Mix II 

NM2001/NM2002/NM2003

40rnxs/400rnxs/2000rnxs

NGS amplification library preparation

DSPath NGS Multiplex PCR Master Mix

K030-A/K030-B

80rnxs/400rnxs

NGS amplification library preparation

DSPath NGS Multiplex PCR Master Mix 

K031-A/K031-B

80rnxs/400rnxs